Register for free to join our community of investors and share your ideas. You will also get access to streaming quotes, interactive charts, trades, portfolio, live options flow and more tools.
“ saying to be cautious “
“and challenged everyone to a fight who dared express caution“
Flip flopping with the winds of emotion isn't good!
I heard somewhere …Be careful who you follow
James 8…..
“A double minded man is unstable in all his ways”
Not to mention saying to be cautious …
odd considering he’s Mr YMIGH himself…the one who declared KBLB a guaranteed sure thing, and challenged everyone to a fight who dared express caution.
Unbelievable
Why ask another poster, when you can address the direct source?
My stance hasn't changed.
THERE ARE NO GUARANTEES ALL GOES WELL, IN THE EVENT THEY ARE ABLE TO SCALE UP.
NOT EVEN THEIR SERICULTURE EXPERTS CAN PREDICT WITH 100% CERTAINTY.
ALL THE PROMOTIONAL PIECES IN THE WORLD, CAN'T SPEAK TO AN ABSOLUTE EITHER....
THERE...
FEEL BETTER NOW? 🤣😂🤣
M4N Wasn’t it a few months ago pushing the narrative that the technology was flawed and not going to happen his thing? And now it’s a different angle lol
That’s easy. They PRd something about Kraig Labs. It’s still on their website.
You poor thing. Have you got your number of posts back up to a good number?
You keep forgetting to login to this one after using one of your other names.
Your other personalities aren’t as unhinged. You should stick to those.
The exuberant optimism is definitely detected but in all reality, if this show does have a
chance at being a profitable player in the selected industry, it's going with a blend; limited
edition, first.....
It's the evolution of the process:
Crawl, walk, then run.
CHECK OUT POLARTEC'S LATEST PARTNERS AND DECIDE FOR YOURSELF IF THE POTENTIAL EXISTS FOR A VENTURE OF SORTS:
PARTNERS
If we are to look forward to the "WILD WILD WEST" OF PERSONAL ATTACKS...
It will FURTHER dilute this board; making KBLB'S DILUTIVE TACTICS PALE IN COMPARISON, WHEN IMPLEMENTED...
UNLESS THAT IS THE PLAN (GOING FORWARD) ALL ALONG.
Will be awaiting their announcement...
With cautious "neutralism."
Translation: yarns and Pima cotton blends made with BAM1 are on the way.
Not with this specific (BAM I) developed.
Not all strains are created equal.
They’ve already made the yarns and the silk and Pima cotton blend. What additional prototypes are you suggesting they will make?
Sorry Rayo...
Not your svengali either.
If the business relationship is terminated, now certainly wouldn't be the opportune time to divulge such an occurrence.
In the business world, "on again, off again" relations happen more often than one may realize.
STAY TUNED...
(sorry you were FORCED to read this Z06 Man) 😂🤣😂
At some point, they'll decide to produce actual prototypes; just to be certain all quality control parameters are met.
I believe this will come before going "full steam ahead" with the pursuit of metric ton production.
The following is the 3rd (!!!) paragraph in the article.
Great article breaking down the RFI! Everything from potential award money to the background of why the need for it. Enjoy! Especially considering the author leads with a "spider silk" mention. 😁
https://breakingdefense.com/2023/03/pentagon-seeks-to-jumpstart-nascent-biomanufacturing-industry-with-new-strategy/
Pentagon seeks to jumpstart ‘nascent’ biomanufacturing industry with new strategy
DoD’s new Biomanufacturing Strategy and an accompanying RFI from industry both aim to help companies using biological processes to make potentially revolutionary materials — and to keep them from moving offshore like semiconductors.
By SYDNEY J. FREEDBERG JR.on March 23, 2023 at 4:46 PM
WASHINGTON — Imagine body armor made from spider silk, three times the strength of Kevlar. Frontline airfields made of self-healing concrete, full of bacteria that grows crystals to fill cracks as fast as they appear, or using cement alternatives grown like coral to simplify supply lines. Tiny, wearable sensors that use microbes to detect natural toxins or biochemical attacks. Anti-corrosive coatings for aircraft or Navy ships. A host of novel medicines.
These are just some of the applications researchers are actually working on in the field of biomanufacturing, the use of biological and biologically inspired processes to build new materials. Today, it’s a young but promising field, with lots of intriguing experiments but little that’s on a commercially or militarily useful scale.
Spider silk is one of the strongest materials in nature, for instance, but you can’t farm spiders the way you do silkworms — while silkworms eat mulberry leaves, spiders will eat each other — [WAIT FOR IT...]
While staying in denial of progress towards that? That must be the new counterintuitive investing Buffett speaks of. 🤣
Glad that photo works for you.
I'm awaiting the visual (and verbal) results of wearable prototypes.
KBLB claimed to have produced shirts and the like years ago...
Yet here they remain; sans revenues.
that Polartec pr came out before the company was bought out by some privately held 100 yr old company run by old, staid, bible thumpers...
not sure Polartec was a good fit after that...
Maybe they will exceed one metric ton by the end of the year. Wouldn’t that flip Webby’s burger?
They exceeded my expectations when they showed this:
Now we just need them to hit their metric ton target by the end of 4Q. Seems easier and smoother with VSA and Kumar on board.
One thing I haven’t seen is any public comment by Walter Wee. So either Kings and KBLB have called a lid on Spydasilk until product launch and reveal, or KBLB is planning on giving silk to another customer in the near-term. Never a dull moment around here that’s for sure. I can’t wait to see what happens next.
I hope you didn’t watch any of that channel’s other videos. I made the mistake of watching the scorpion farm after I watched the one on silkworm farming. I don’t think I’ll be able to sleep tonight. If I was forced to choose between eating a silkworm or a scorpion, I think I would rather starve to death. Hopefully before I die we see KBLB over $5 and I can be buried in a Spydasilk suit and tie.
No worries. Sounded outrageous to me. Was looking for clarification.
From the post I replied to. It said KBLB HAD 10 kilos of inventory and they valued it at just under $6600 on the financials.
You are wrong! You bashers keep forgetting that it took KBLB five years to enter Vietnam, and the company lost four years due to COVID. So, subtract nine years, and KBLB has amazingly come a very long way. Unfortunately, this will all fall on deaf ears, and you and the other brain dead bashers will lose out on monumental gains from this goldmine. When we are bought out, you and others will be sitting there shaking your head and kicking yourself in the ass for not buying. Oh, well, I tried. Remember, you can take a horse to water, but you can't make him/her drink. GLTY GO KBLB!
After further review, I’m not dismissing it as old news necessarily. The copyright year is 2024.
I believe they just referenced the polartec PR from 2019. The language is drawn from that PR yet the page was made this year.
It would be fantastic if Polartec were the recipient of the initial batches. They had been working with KBLB since 2016 before the PR.
(I hope Kings is out of the picture)
Here’s the Polartec PR: https://www.polartec.com/news/kraig
Nice video. Thanks.
They aren’t pulling the wool over our eyes truth…. Been there done that
I do hope I’m wrong, we are wrong. Still have skin in the game. But playing with found money.
I don't see anything about homozygous parental lines or India's top sericulture expert. Don't be so lazy. Keep looking.
Better yet, call the police on me. LMAO
It isn't the first time throughout its (debatable) sordid history, the company PR'd production exceeding expectations...
I believe the exact verbiage was "meeting or exceeding expectations."
SEE FOR YOURSELF...
LANSING, Mich., Feb. 25, 2013 (GLOBE NEWSWIRE) — Kraig Biocraft Laboratories, Inc. (OTCQB:KBLB) (the “Company” or “Kraig”), the leading developer of advanced spider silk based fiber technologies, announced today that the first two stages of its Monster Silk™ pilot production program (“program”) are running ahead of schedule and that both stages of the program are on track to meet or EXCEED the program’s goals.
The production capacity for the first two stages EXCEEDS the Company’s prior maximum capacities by a factor of four or five.
The program is designed to be the basis of the Company’s large scale commercial production of its recombinant spider silks. The program is designed to run in three stages; with each successive stage launching when the previous stage’s data indicates that the program is reaching, or EXCEEDING, its benchmarks.
The current data supports the continued fast-tracking of the program, as the second phase is developing on track, SURPASSING the trial’s initial phase in terms of capacity and success rates. The first stage continues to run AHEAD OF EXPECTATIONS and is already moving to an advanced state.
“So far the pilot production program has EXCEEDED OUR EXPECTATIONS AND IS CONFIRMING THAT OUR PRODUCTION MODEL IS FULLY SCALABLE, as Phase II surpassed the program’s first phase, despite the fact that it’s a much larger trial,” said Kraig founder and CEO, Kim Thompson. “From the beginning, this has been an ambitious program, which now appears to be rewarding our efforts. We are continuing to rapidly advance through stage two of the production program.”
KRAIG BIOCRAFT LABORATORIES PROVIDES UPDATE ON THE MONSTER SILK(TM) PILOT PRODUCTION PROGRAM’S SECOND STAGE; PROGRAM IS ACCOMPLISHING ITS TARGETED MILESTONES AND REMAINS WELL AHEAD OF SCHEDULE
How Japanese Farm Millions of SilkWorm for silk - Silk cocoon harvest and process in Factory
Video:
PR's from Kimbo and crew are nothing but fluff. Read the latest 8+ years of them and prove me wrong.
I wonder if the Australian connection means anything…
https://www.dcgroup.co/portfolio/cambodia-s-no-1-real-estate-marketplace-since-2009
PARTNER OPPORTUNITIES
With our focus on a corporate audience, whether company owners and managers or those travelling to Cambodia for business purposes,
B2B CAMBODiA offers a range of promotional programmes for companies and individuals offering products and services.
For more details on how to partner with us, please contact us through our email sarvinoz@b2b-cambodia.com.
ABOUT B2B
If BAM1 silkworms are bigger……. why not make them into burgers and fries too? I wonder if Kim has ever been approached by food companies to increase the size of silkworms for food purposes?
Check this out:
"SILK FOOD" by Ellie Turns Silkworms Into Burgers, Dips, Fries & More
https://hypebeast.com/2020/2/silk-food-ellie-hiroshi-fujiwara-japan-silkworms-sustainable-plant-based-alternative
And that’s how you use the entire buffalo!
I just found this article too. Nice to see it got picked up on a Cambodia-related business news site.
I figured out your source and have corrected their mistake. Sorry!
You made a mistake. The inventory shown in the Q3 2022 10-Q was listed at $6,580. At the end of 2023, the inventory increased to $6,884, an increase of $304.
KBLB showed a hank of silk in the December 2023 photo. We don’t know the weight of that hank. If it was 10 kilos, that suggests KBLB priced the silk at about $30/kilo.
The implication is that KBLB produced about 219 kilos in all of 2022, 10 kilos during all of 2023, and no additional silk in Q1 2024.
Pricing it at $30/kilo does not imply that is the sales price of the silk—only the production ‘cost.’ It appears that KBLB paid GSS $100,000 to produce 219 kilos of silk.
These numbers suggest why KBLB abandoned their previous strains and are now hoping for a base hit or better with Bam-1.
Stay tuned… the KBLB way.
No one will pay $660,000 for a metric ton of Bam-1. Not even the military. I don’t know where you got your numbers, but $160,000 to $240,000 would be about all the market could bear.
Where did you get that figure?
Followers
|
638
|
Posters
|
|
Posts (Today)
|
31
|
Posts (Total)
|
276819
|
Created
|
05/04/08
|
Type
|
Free
|
Moderators MU_Redskin1 gimmegimmeminemine TRUISM EOT WebSlinger |
Email: corporate@KraigLabs.com
KRAIG LABS WEBSITE FOR INVESTORS
Quarterly and Annual Reporting to the SEC is available on the Company's Website and EDGAR.
* Financial Statements * SEC Filings *
Outstanding Shares as of January 12, 2023
For issues or questions relating to share certificates or the transfer of securities please contact the company's transfer agent:
Olde Monmouth Stock Transfer Co., Inc.
200 Memorial Pkwy.
Atlantic Highlands, New Jersey 07716
Phone: (732) 872-2727
(since August 14, 2013)
Kraig Biocraft Laboratories, Inc. (KBLB) is the first company with a commercially feasible spidersilk to be mass produced.
Kraig Labs is a biotechnology company focused on the development of commercially significant high performance polymers and technical fibers. Kraig Lab's focus has been on the production of a transgenic silkworm incorporating specific gene sequences from the golden orb weaving spider. These specific gene sequences inserted are to enable the silkworm to spin a new recombinant fiber which incorporates spider silk proteins. With the scientific breakthrough announced on September 29, 2010, Kraig Labs is now working to commercialize the transgenic silkworms to compete in the garment industry silk market. The value for the chinese raw silk market alone is 3-5 billion per annum. With the creation of 20 seperate transgenic silkworms, all with unique properties, Kraig Labs is now working at an accelerated pace to build upon their first generation transgenic organisms to develop their second generation of transgenic silkworm incorporating spider silk proteins. The scientists nearly doubled the strength of the silkworm with these specific spider gene insertions. Their second generation of transgenics are expected to be complete in 2011. These second generation organisms are to be compared with the strength, flexibility and resiliency of the native spider in which the gene sequences are derived from. These fibers which will match the strength of spider silk are expected to compete in the technical textiles market valued in excess of 120 billion per annum. The 3rd generation organisms are currently in the planning phase. These organisms are expected to spin fibers exceeding the strength of native spiders and may incorporate gene sequences that release an antibiotic, or to help reduce scarring with use in bandages.
Kraig Biocraft Laboratories has a sponsored research and development program with the University of Notre Dame, and the University of Wyoming. The genetic work is occurring at the University of Notre Dame, headed by Dr Malcolm Fraser, Phd. The gene sequences are derived from Dr. Randy Lewis's(University of Wyoming) patented gene sequences of the golden orb weaving spider. Kraig labs is paying for all expenses incurred for this research and development program, and thus Kraig Labs has exclusive global commercialization rights with the technologies developed, including methods, organisms, and fibers produced.
MANAGEMENT
Kim Thompson, Founder and CEO
As the CEO of the company, Mr. Thompson is the only member of the scientific advisory board who is also
a part of the corporation's management. His formal education lies in the fields of economics and law.
He received his B.A. in Applied Economics from James Madison College at Michigan State University.
He received his Juris Doctorate from the University of Michigan Law School in 1994.
Mr. Thompson founded Kraig Biocraft Laboratories in his pursuit of the development of new biotechnologies
with industrial applications. As chairman of the scientific advisory board, he brings a unique perspective,and
acts as the primary liaison between the advisory board and the corporation.
Mr. Thompson brings a wealth of experience in business management and consultation to Kraig. Following
the completion of his undergraduate degree, Mr. Thompson joined California Craftsman, Inc. as a
Vice-President with primary responsibility for both marketing and human resources.
Kim Thompson was the director of business development at Franchise Venture Partners, LLC. He subsequently
joined the firm of Shearson, Lehman, Hutton where he specialized in equity trading and research of small cap
companies. Mr. Thompson received the highest series seven score for all Shearson brokers in his class nationwide.
His experience in those small cap equity markets has proven to be invaluable both in his legal and business successes.
Prior to becoming a public company CEO, Mr. Thompson was the founder and senior litigation partner in a California
commercial law firm where he worked as corporate and litigation counsel to privately held and public companies.
His many accomplishments in corporate law include winning and collecting in full what his firm believes to have been
the largest award of lost profits in a California commercial arbitration up to that time. An important part of his work was
winning victories on behalf of corporate clients in disputes over intellectual property and distribution rights. He has
represented business clients ranging from small start ups and micro caps to Fortune 100 companies.
With a background in business leadership and in advising public and private corporations, Kim Thompson continues
to bring a unique perspective to the successful management of business. His extensive business and legal background
enables him to create practical solutions to business problems and seize opportunities for growth.
Mr. Thompson is a member of the Triple Nine Society for persons with documented genius level IQs (having tested above
the 99.9th percentile). He is also active in the realm of science and invention where he has to his credit a number of
provisional patent applications including innovations in the areas of biotechnology, organic polymers, genetic engineering
and magnetic field manipulation, among others.
Mr. Rice has over 13 years’ experience growing development stage businesses with a focus on technology development, commercialization, and go to market strategies. Mr. Rice holds a B.S. in Chemical engineering from Michigan Technological University.
Prior to joining Kraig Biocraft Laboratories Mr. Rice was the Director of Advanced Technologies for Ultra Electronics, AMI. In this role, Mr. Rice was responsible for the identification, capture, and execution of new technology programs. During his tenure with AMI, Rice secured more than twenty five million dollars in funded development programs from the US Department of Defense which his team successfully leveraged into commercially viable spinoff products. Mr. Rice was also responsible for technical sales, marketing, and promotion of AMI’s products and capabilities. Rice joined AMI as the third full time employee and helped to lead the organization through its rapid growth and ultimate acquisition by Ultra Electronics in 2011.
Earlier in his career Mr. Rice developed unique advanced manufacturing techniques, established and trained a production staff, led engineering development, authored numerous technical papers, and is a recognized subject matter expert. Mr. Rice holds 5 issued patents and numerous provisional patents.
Mr. Rice brings a history of transforming revolutionary ideas into viable commercial products.
Mr. Rice is currently completing his Masters of Business Administration through the Executive Program at the Eli Broad College of Business: Michigan State University.
Despite the huge potential of genetically modified animals outside of laboratory research, commercialisation of these animals has been extremely limited. Numerous factors, including negative consumer perception, regulatory hurdles, and limitations inherent to classical GM technologies, have kept the majority of GM animal applications within the realm of academic research. However genome editing using zinc finger nucleases could help develop new markets for the future commercialisation of GM animals.
Genetic modification is commonplace throughout the life sciences sector, from fundamental research to pharmaceutical testing. GM cellular and animal models are valuable tools for the study of many chronic diseases, the testing of pharmaceutical compounds and the development of new therapeutic strategies. Genetic modification also offers great benefits in vaccine and biopharmaceutical manufacturing, which rely heavily on the use of GM organisms for biomolecule design and production. Modifying the genome of an organism or cell line allows the incorporation of target biomolecules in specific biological contexts, as well as the transfer of a gene product from a low-producing organism to one that can produce on a commercial scale. These applications have been widely accepted for many years, with countless GM organisms approved for medical manufacturing applications by drug regulators in all major countries. Despite this widespread success within the research and pharmaceutical sectors, the use of GM organisms outside of these markets has been limited.
Despite the lack of broad acceptance for most commercial applications of GM animal products, this technology has been able to gain traction in a few market sectors. The most obvious application has been the commercialisation of transgenic animals for the production of biomolecules for therapeutic use. Cattle, sheep and goats have been used for large-scale production of antibodies, steroids and hormones - most notably insulin - for many years. In 2009, GTC Biotherapeutics received US FDA approval for bioproduction of a recombinant human antithrombin. This product - ATryn - is extracted from the milk of transgenic goats, and is the first approved biopharmaceutical to be produced using genetically engineered animals. Although this is a significant breakthrough for the commercialisation of GM animals, it is still within the pharmaceutical industry, and is a natural progression of existing cell-based technologies. Of potentially greater commercial interest is the extension of genetic engineering outside of this sector, into areas such as food production, textiles and even companion animals.
GM crops have been available in many countries since the early 1990s, and numerous cash crops - including sugar beet, soybean, corn and tomatoes - have been modified to improve resistance to disease, increase the rate of growth or enhance nutritional value. However, cultivation of these transgenic crops is generally tightly regulated, particularly within the European Union, and this, together with negative public opinion, has limited the more widespread development of GM technologies.
Similar to GM crops, many of the animals currently under development are intended to confer disease resistance, an application particularly suited to the use of zinc finger nuclease (ZFN) technology. Many diseases can be treated by the targeted deletion or modification of a host gene. With ZFNs, these targets can be modified with no footprint of genetic engineering. Due to the high costs of raising livestock, another area of focus in developing commercial GM animals has been increasing the rate of growth or size of animals. Among the first GM animals likely to be launched is a fast growing salmon from AquaBounty. The AquAdvantage Salmon is designed to reach market size in half the time of a wild type salmon, reducing costs for fish farmers and limiting the environmental impact of salmon farming by avoiding the need for ocean pens.
Although genetic engineering of animals for food is primarily driven by economic pressures, GM technologies have also been used in the companion animals market. In this sector, genetic modification can be used for practical purposes - such as the creation of hypoallergenic animals or the correction of heritable congenital defects which have arisen though inbreeding - or for purely cosmetic purposes, such as GloFish. The first example of a GM pet, GloFish are fluorescent zebrafish (Danio rerio) that have had genes encoding naturally fluorescent proteins (GFP, YFP, RFP) inserted into their genome. Developed by a group at the National University of Singapore, GloFish were originally created to develop live detection systems for water pollution. They were introduced as pets in the United States in 2003 following over two years of extensive environmental research and consultation. In Europe however, the sale and possession of GloFish is prohibited by rigorous legislation concerning the use of GM technologies.
By allowing precisely targeted insertion of spider genes and concomitant removal of endogenous silkworm silk genes at the same locus, ZFN technology offers the potential for development of transgenic silkworms which will produce native spider silk at commercially viable levels
Perhaps even more interesting from a commercial perspective is the use of GM animals in the manufacture of textiles. Silkworms - actually the larval form of the silkmoth Bombyx mori - have been used for the production of silk for thousands of years, with natural silk still produced by the cultivation of silkworms today. Silkworm cocoons are unwound to create linear silk threads, then re-spun into textiles in much the same way as cotton. Although the applications of silkworm silk are numerous, due to their unique physical and chemical properties, there is also widespread interest in the silks of several other insects.
Spider silk, in particular, offers numerous possibilities within the technical textiles industry, due to its incredible tensile strength and elasticity; characteristics which have not yet been replicated in synthetic materials. Like all insect silks, spider silk fibres consist of repetitive units of protein crystals separated by less structured protein chains. The exact properties and composition of each spider silk vary with its intended function. Major Ampullate or dragline silk, for example, is relatively hydrophobic with very high tensile strength and toughness, as it is used to form the outer rim and spokes of a web. In contrast, hydrophilic capture spiral silks, which form the inner structures of the web, are sticky and highly elastic to effectively entrap prey. This high degree of variability offers enormous potential for the textiles industry, raising the possibility of tailoring the properties of silk to create advanced technical fabrics, for applications such as bulletproof vests, parachute canopies and automobile airbags; biomedical applications, including sutures and tendon and ligament repair; new fabrics, for sportswear and clothing; and even microelectronics.
Although the use of spider silks for microsutures has recently been reported, more widespread application of spider silk technologies is currently limited by the difficulty in producing silks on a commercially viable scale. This is due to the difficulties of rearing spiders in large numbers, due to their highly territorial and cannibalistic nature. As a result, the harvesting of spider silk fibres is extremely time consuming and labour intensive, with production of the only known spider silk garment - an 11 foot by 4 foot shawl made from golden orb spider silk - taking 150 people over five years to produce and costing in excess of £300,000!
.
To overcome these limitations, and allow future development of spider silk technologies, an alternative strategy for spider silk production is required. This makes spider silk production an obvious candidate for genetic modification, inserting spider silk genes into the genome
of other silk-making insects for bioproduction. For example, random insertion of orb spider silk genes into silkworms has allowed production of hybrid spider/silkworm silk using traditional silkworm farming strategies. The resulting hybrid silk contains approximately 10% spider silk
and has greater strength and durability than native silkworm silk, raising the possibility of using transgenic silkworms to produce pure spider silks.
Though straightforward in principle, the exchange of native silkworm genes for spider silk genes, alongside more widespread exploitation of genetic engineering, has been limited by the inherent restrictions of conventional GM technologies.
The generalised process of modifying an organism requires several capabilities, including:
While many different techniques exist for accomplishing each of these steps, most GM technologies offer a compromise between the efficiency of the technique and the ability to accurately and precisely target the locus of interest. Viral genomic delivery technologies effectively deliver nucleic acids to cells and organisms, but fall short on ability to target specific regions of the genome, generally only allowing random insertion of genetic material. In comparison, transposase technologies allow a greater degree of targeting, but leave unwanted traces of exogenous DNA in their wake. Other methods involve the introduction of naked DNA into the cell, which results in insertion into the genome at very low frequencies, usually at random, limiting this approach to organisms that can be economically cultivated at high densities and screened in large numbers. Simply put, most techniques for genetic manipulation are random, inefficient and leave a 'footprint' of foreign DNA. While this is usually tolerated in basic research, it is not acceptable for most commercial applications, and has been a major hurdle for GM animal technologies to date.
The advent of zinc finger nuclease (ZFN) technology represents a significant breakthrough for commercialisation of GM animal products, offering precisely targeted, efficient genome editing for the first time. Commercially available through Sigma Life Science under the CompoZr brand, this technique can be used to create permanent and heritable changes to an organism of interest.
This high degree of variability offers enormous potential for the textiles industry, raising the possibility of tailoring the properties of silk to create advanced technical fabrics
ZFNs are a class of engineered DNA binding proteins that facilitate targeted editing of the genome by creating double-strand breaks at user-specified locations. These breaks stimulate the cell's natural DNA repair mechanisms - homologous recombination (HR) and non-homologous end joining (NHEJ) - which can be exploited to achieve rapid and permanent site-specific modification of the desired genes. While HR can be used to insert foreign DNA sequences, NHEJ allows the cell's natural processes to create precisely targeted mimics of natural mutations which leave no traces of foreign DNA. Unlike previous techniques, ZFNs offer excellent sequence specificity, binding 24 to 36 base pair target sequences to virtually eliminate off target effects, and are able to achieve modification rates exceeding 20 %, well above rates for most other technologies.
The technique is already being used to create transgenic silkworms for spider silk production. By allowing precisely targeted insertion of spider genes and concomitant removal of endogenous silkworm silk genes at the same locus, ZFN technology offers the potential for development of transgenic silkworms which will produce native spider silk at commercially viable levels.
GM technologies have revolutionised the research world and have great potential in a variety of commercial applications, but have been limited by the inherent restrictions associated with historical GM technologies. The main drawback of these technologies is their inability to accurately and efficiently target genes of interest, instead relying on random insertion of genetic material into host genomes. These limitations result in the need for extensive and costly screening to identify animals with correct transgene expression (without compromising the viability of the animal), and also result in the co-expression of both the transgene and native homologues already present.
The advent of ZFN technology signifies the beginning of an exciting new chapter in the world of genetic modification, allowing precise, targeted and efficient genome editing for the first time. Production of native spider silk using transgenic silkworms is just one example of the potential commercial applications of this innovative technology, taking us one step closer to the reality of industrial scale biomanufacturing and paving the way for an entirely new spectrum of environmentally friendly materials.
Authors:
Joseph Bedell and Brian Buntaine
Commercial Animal Technologies Group, Sigma Advanced Genetic Engineering (SAGE) Labs, Sigma Life Science
HEADLINES FOR KRAIG BIOCRAFT LABORATORIES / (KBLB):
PHOTOS FROM VIETNAM POSTED JULY 6, 2018
ANN ARBOR, Mich., – January 7, 2019 –Kraig Biocraft Laboratories, Inc. (OTCQB: KBLB) (“Company”), the leading developer of spider silk based fibers, announces today that it has successfully delivered the first two shipments of its highly specialized silkworms, which produce a silk with the physical characteristics of spider silk, to Vietnam.
Today’s announcement is the culmination of more than 5 years of work, and challenging negotiation, with the government of Vietnam. The silkworms from these first two shipments have already hatched and are now enjoying a fresh mulberry diet, so, for the first time in history, the global demand for spider silk materials has a viable, cost effective, and scalable solution.
“The dream of commercializing our powerful technology is now materializing. This marks a dramatic leap forward in Kraig Labs’ business plan for commercial production and mass marketing of cost effective recombinant spider silk, and becomes the foundation for an entirely new industry,” said, COO, Jon Rice. “To our long-term shareholders, who have taken this journey with us, as well as our team in the US and Vietnam, who have worked tirelessly to make this dream a reality, I cannot thank you enough. As we start the New Year, full of new opportunity, we truly have something incredible to celebrate.”
The Company has been working with leading sericulture experts, biotechnology institutions, and governmental agencies, in Vietnam, to further boost the silk industry with our revolutionary technology. Kraig Labs is currently finalizing renovation plans for a ~46,000 square foot production factory in Quang Nam Province, Vietnam.
“Our plan has always been to bring our technology to the silk producing regions of the world for rapid scale-up,” said, CEO and Founder, Kim Thompson. “Today we see the fruits of that effort. With its massive silk infrastructure and production capacity, Vietnam is an ideal location to launch our technology scale-up. Congratulations to our team and shareholders, as we prepare for the realization of large scale production.”
\
\
Volume | |
Day Range: | |
Bid Price | |
Ask Price | |
Last Trade Time: |